SUMMARY

During the last decade, a number of the known oncogenes and tumor-suppressor genes have been shown to be altered in lung tumors. The tumor growth and progression of the lung cancer strict1y depend as another neoplasm on an imbalance between cellular proliferation and death, the wich is controlated by altered cellular genes. The oncoproteins of the human papillomavirus (PVH) interacting many times with the products of tumor-suppressor genes characterization events of carcinogenetic process of this neoplasia.

Objective: To evaluate the behavior of oncogenes and tumor-suppresor genes and its possible association with human papillomavirus oncoproteins in the lung neoplasia of two patients with old history of recurrent respiratory papillomatosis positive to HPV-DNA.

Materials and Methods: We studied paraffin embedded biopsies of two patients with diagnosis of 1ung carcinoma. The expression of the biologic parameters P53, Bcl-2, Bax and PCNA proliferative activity were detected applying immunohistochemistry, and the viral oncogenes E6-E7 of the HPV-6, 11, 16, 18, 31, 33, and 35 by means the polymerase chain reaction PCR using type specific primers and restriction maps.
Results: In both cases studied were detected HPV-DNA, in one of them was identified multiple infection to the HPV-11, 16, 18 and 31, and in the other case the types 6, 11,16 and 18. On1y one of the cases was immunoreactive for the four markers P53, Bcl-2, Bax and PCNA simultaneously. The second cases was only positive for PCNA and Bax.

Conclusions: Our results support the possibility that some of HPV might play an important role in the development of 1ung neoplasm with previous history of recurrent laryngeal papilomatosis positive HPV-DNA, and suggest to the proteins of oncogenes and tumor-suppressor genes in lung cancer, as clinic potential of this cellular and viral markers, to be careffully applyed in fields of diagnosis and prognostic, and can in the future had an iniportant role likely in the therapy and prevention of patients with susceptibly showed, for the which is necessary major studies.

Key words: lung cancer, HPV, p53, bcl-2, bax, PCNA.

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