Rev. peru. biol.   2003; 10 (1): 67-77

 

ENZYMATIC DEGRADATION OF CELLULOSE FOR THERMOPHILIC ACTINOMYCETE: ISOLATION, CHARACTERIZATION AND CELLULOLYTIC
ACTIVITY DETERMINATION

Pablo Ramírez y Juana María Coha *


SUMMARY

One hundred and forty five cellulolytic thermophilic actinomycete strains were isolated from 71 compost, soil, hay and dung samples. Streptomyces sp. (50,63%), Thermomonospora curvata (15,82%), T. chromogena (13,92%), and other species were identified. Endoglucanase, exoglucanase and b-glucosidase activities were evaluated from 10 cellulolytic actinomycete strains. Among these the Streptomyces sp. 7CMC10 strain showed the biggest activity levels corresponding to 20,14; 2,61 and 5,40 UI/mg of protein, respectively.

Key words: Actinomycetes, cellulases, thermophilic, Streptomyces, Thermomonospora, biotechnology

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