Figure1. Rhodamine-Phalloidin stainig of microfilament in dividing P. purpuratus eggs. Arrow head, fluorescent foci; arrow, difuse fluorescence; PB, polar body (X400);
Figure 2. Staining of the same preparation with antitubulin monoclonal antibosy and with DAPI dye. C, cilius; V, vellum; arrow, nucleus (Dapi) (X400)
Figure 3. Unfertilized P. purpuratus egg stained with DAPI. VM, vitelline membrane; YC, jelly coat; arrow, nucleus (X400).
Figure 1´-3: Schematic drawings to facilite the comprehension of the legends of the ilustrations